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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become noticed in several tumors, which results in chromosome instability, and thus may perhaps contribute to tumorigenesis. This protein was observed to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial position in marketing mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are explained. Two added splice variants ended up documented but thorough nucleotide sequence details will not be yet out there.
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Blocking peptides are peptides that bind especially into the target antibody and block antibody binding. These peptide commonly incorporates the epitope regarded through the antibody.
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This manual outlines further more attainable optimization of mobile and immunoassay parameters to make sure the very best final results are...
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This guide outlines further more feasible optimization of mobile and immunoassay parameters to make certain the very best results are...
Antibodies certain to the blocking peptide not bind for the epitope within the focus on protein. By evaluating the staining with the blocked antibody vs . the antibody by yourself, one can see which staining is certain.
Goal Details Cyclin E belongs to your highly conserved cyclin household, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation styles which contribute into the temporal coordination of every mitotic party. Cyclin E kinds a fancy with and capabilities like a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary and it is degraded as cells progress by way of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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